#373 – Thyroid function and hypothyroidism: why current diagnosis and treatment fall short for many, and how new approaches are transforming care | Antonio Bianco, M.D., Ph.D.

Nov 17, 2025 Episode Page ↗
Overview

Dr. Antonio Bianco, a physician-scientist and expert in thyroid physiology, discusses the complex biology of thyroid hormones, the limitations of TSH-only diagnosis, and the controversies surrounding hypothyroidism treatment. He explores the roles of deiodinase enzymes, genetic variability, and the potential benefits of combination therapy over T4 monotherapy.

At a Glance
27 Insights
2h 20m Duration
18 Topics
7 Concepts

Deep Dive Analysis

Introduction to Dr. Antonio Bianco and Thyroid Research Focus

Fundamental Biology of Thyroid Hormone Production and Action

Role of Deiodinases and Reverse T3 in Thyroid Hormone Regulation

Impact of Fasting on Thyroid Hormone Levels and Metabolism

Hypothalamus-Pituitary-Thyroid Axis and TSH Regulation

Importance of Understanding Thyroid Physiology for Treatment

Thyroid Hormone Testing: Free vs. Total Levels and Assay Limitations

Genetic and Sex-Based Variability in Thyroid Regulation

Hyperthyroidism: Causes, Symptoms, Diagnosis, and Treatment Options

Hypothyroidism: Autoimmune Causes and Diagnostic Biomarkers

Thyroid Hormone Replacement Therapy: T4 Monotherapy vs. Combination

Desiccated Thyroid Extract: History, Pros, and Cons

Mortality and Comorbidities in Hypothyroidism Treatment

Debunking Misconceptions in Hypothyroidism Diagnosis

Challenges with Compounded Controlled-Release T3

Risks of High-Dose Iodine Supplementation

Managing Subclinical Hypothyroidism and Age-Related TSH Changes

Future Directions in Thyroid Diagnosis and Treatment

T4 (Thyroxine)

T4 is the primary pro-hormone secreted by the thyroid gland, containing four iodine atoms. It is largely inactive and serves as a circulating storage form with a long half-life of about eight days.

T3 (Triiodothyronine)

T3 is the active form of thyroid hormone, produced by removing one iodine atom from T4. T3 binds to cellular receptors with high affinity, regulating gene expression and metabolism, but has a short half-life of about 12 hours.

Deiodinases (D1, D2, D3)

These are enzymes that regulate thyroid hormone activity at the tissue level by either activating T4 into T3 (D1, D2) or inactivating T4 into reverse T3 (D1, D3) and T3 into T2 (D3). D2 is particularly efficient, producing about 80% of T3 made outside the thyroid gland, especially in the brain and hypothalamus.

Reverse T3 (rT3)

Reverse T3 is an inactive form of T3 produced when an iodine atom is removed from the inner ring of T4. Its production increases when the body aims to reduce thyroid hormone action and conserve energy, such as during fasting.

Hypothalamus-Pituitary-Thyroid (HPT) Axis

This is a regulatory system where the hypothalamus releases TRH, stimulating the pituitary to release TSH, which then stimulates the thyroid gland to produce T4 and T3. This axis maintains stable thyroid hormone levels in the blood, but local tissue T3 levels can vary significantly due to deiodinase activity.

Hashimoto's Disease

The most common autoimmune cause of hypothyroidism, where the body's immune system produces antibodies (e.g., TPO antibody) that attack and destroy the thyroid gland. This leads to reduced thyroid hormone production and is typically treated with hormone replacement.

Subclinical Hypothyroidism

This condition is characterized by an elevated TSH level (e.g., 5-10 mIU/L) but normal free T4 levels. Patients may or may not experience symptoms, and the decision to treat depends on factors like age, symptoms, and antibody status.

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What is the primary function of the thyroid gland?

The thyroid gland traps iodine from the blood to produce thyroid hormones, primarily the inactive pro-hormone T4, which it stores and slowly releases into circulation.

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How does the body activate thyroid hormone?

T4 is activated into the biologically active hormone T3 by deiodinase enzymes (D1 and D2) that remove one iodine atom, primarily in peripheral tissues like the liver, heart, and brain.

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What is reverse T3 and what is its role?

Reverse T3 (rT3) is an inactive form of T3 produced by deiodinase enzymes (D1 and D3) when an iodine atom is removed from the inner ring of T4, serving as a mechanism to reduce thyroid hormone action and conserve energy, such as during fasting.

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Why is measuring free T3 and free T4 preferred over total T3 and total T4?

Most T3 and T4 in circulation are bound to proteins and inactive; only the tiny "free" fraction can enter tissues and exert biological effects. Free hormone levels are more accurate for diagnosis as they are not affected by changes in binding proteins.

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Why are current T3 and reverse T3 assays often unreliable?

The existing immunoassay methods for T3 and reverse T3 are not gold standards and have high variability, especially at lower levels, making them less accurate than mass spectrometry, which is not routinely available.

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What are the main causes of hyperthyroidism?

The two major causes are Graves' disease (an autoimmune condition where antibodies stimulate the thyroid) and hyperfunctioning nodules (adenomas) that autonomously produce excess thyroid hormone.

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What is the most common cause of hypothyroidism?

The most common cause is Hashimoto's disease, an autoimmune condition where the body's immune system attacks and destroys the thyroid gland.

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Why is TSH normalization not always sufficient for treating hypothyroidism?

While TSH normalization is the primary goal of conventional therapy, many patients on T4 monotherapy still experience symptoms and have increased mortality from cardiometabolic diseases, suggesting incomplete restoration of euthyroidism at the tissue level.

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What are the risks of high-dose iodine supplementation?

Excessive iodine intake can trigger autoimmune thyroid disease by increasing the antigenicity of the thyroid, potentially leading to hypothyroidism, and can also induce hyperthyroidism in individuals with existing thyroid nodules.

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How does age affect TSH levels and the diagnosis of hypothyroidism?

After age 50, the upper limit of the normal TSH range increases by approximately one point every 10 years, meaning an elevated TSH in an older individual (e.g., TSH of 8 in an 80-year-old) may be considered normal and not require treatment.

1. Request Mass Spec for T3/rT3

When measuring T3 and reverse T3, request a mass spectrometry (LC-MS) assay instead of immunoassay, especially for low levels, due to its superior accuracy and reliability.

2. Measure Biologically Active T3

Advocate for measuring T3 (the biologically active thyroid hormone) when assessing thyroid function, as its omission reflects an incomplete understanding of thyroid physiology.

3. Rely on Labs, Not Just Symptoms

When evaluating for hypothyroidism, rely primarily on TSH and Free T4 lab results rather than symptoms alone, as many symptoms are non-specific and can be caused by other conditions.

4. Monitor Cardiometabolic Health

If you have hypothyroidism, even when treated, consider it a risk factor for cardiometabolic disease and ensure regular monitoring of cholesterol, LDL, and signs of early cardiovascular disease.

5. Re-evaluate Therapy if Symptoms Persist

If you continue to experience symptoms despite normalized TSH and Free T4, discuss with your doctor that the current therapy may not be ideal and explore further options.

6. Advocate for Better Hypo Treatment

Advocate for improved hypothyroidism treatment, including the development of reliable mass spec T3 assays and pharmaceutical slow-release T3 formulations, to address patient suffering beyond just normalizing TSH.

7. Start Hypo Treatment with T4

For initial hypothyroidism treatment, start with T4 monotherapy (levothyroxine), assuming deiodinases are functioning optimally to convert T4 to active T3.

8. Address Comorbidities First

If T4 monotherapy is ineffective, first rule out and address other comorbidities (e.g., menopause, anemia) causing similar symptoms before considering combination T4/T3 therapy for hypothyroidism.

9. Consider Synthetic T4/T3 Combo

If T4 monotherapy is insufficient, consider synthetic T4/T3 combination therapy, which allows for adjusting the T4 to T3 ratio (ideally around 3.5-4:1) to optimize treatment.

10. Adjust TSH Range with Age

Recognize that the normal TSH range increases with age; for individuals over 50, an elevated TSH (e.g., 6 at 70, 8 at 80) may be considered normal and not require treatment.

11. Screen Pregnant Women for TPO

Pregnant women should be screened for TPO antibodies in the first trimester, and if positive, should be referred to a high-risk obstetrician due to increased risk of miscarriage and prematurity, even without overt hypothyroidism.

12. Avoid High-Dose Iodine

Avoid supplementing with excessive amounts of iodine (above 150-250 micrograms daily), as high doses can trigger autoimmune thyroid disease and hyperthyroidism in some cases.

13. Selenium/Vitamin D for Hashimoto’s

If you have Hashimoto’s disease, consider supplementing with selenium, vitamin D, or other antioxidants to potentially reduce TPO levels and prolong the thyroid’s natural function.

14. Monitor TSH with Family History

If you have a family history of hypothyroidism, a rising TSH, and positive TPO antibodies, monitor TSH every three months to make an early treatment decision, even if minimally symptomatic.

15. Diagnose Secondary Hypo by Low Free T4

If you suspect secondary hypothyroidism (normal TSH with symptoms), ensure your Free T4 is below normal; if so, pursue imaging of the pituitary or hypothalamus to rule out underlying issues.

16. Check FDA for Drug Recalls

Regularly check the FDA website for recalls on thyroid medications, including desiccated thyroid extract and levothyroxine, to ensure the safety and potency of your treatment.

17. Choose High-Volume Surgeons

When considering surgery, especially for specialized procedures like thyroidectomies, seek surgeons who perform a high volume of cases (e.g., at least 100 per year) to ensure expertise.

18. Discuss Post-Iodine Cancer Screening

If you’ve had Graves’ disease and received radioactive iodine treatment, consult your doctor about potential increased cancer risk and discuss appropriate additional cancer screenings.

19. Track Personal Baseline TSH

Keep records of your baseline TSH levels from when you were healthy, as this can serve as a personalized target if you develop hypothyroidism.

20. Ensure Daily Iodine Intake

Consume a reasonable amount of iodine daily through diet (e.g., seafood) or supplemented kitchen salt to support thyroid function.

21. Generic Levothyroxine is Comparable

Do not assume branded levothyroxine (Synthroid) is superior to generic versions; studies show comparable efficacy, and pharmacists may switch to generics without notification.

22. Normalize TSH, Free T4

The primary goal of thyroid hormone replacement therapy is to normalize TSH and Free T4 levels, aiming for biochemical euthyroidism, even if some symptoms persist.

23. Monitor for Co-Occurring Autoimmunity

If diagnosed with one autoimmune disease, be aware of the increased risk of developing other autoimmune conditions and discuss this with your doctor for comprehensive monitoring.

24. T3 Not for Hypo Diagnosis

Do not rely on T3 levels for diagnosing hypothyroidism, as the body prioritizes maintaining normal T3 even in early stages; focus on TSH and Free T4 instead.

25. Trust TSH, Free T4 Assays

Trust TSH and Free T4 immunoassay results, especially when consistently using the same lab, but be cautious with T3 and reverse T3 immunoassay results, particularly at low levels.

26. Master Physiology for Treatment

Deeply understand the underlying physiology of a condition to discern genuine therapies from potentially harmful or ineffective treatments.

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From an evolutionary point of view, the evolutionary pressure is iodine deficiency. So the whole system evolved in a way to preserve iodine.

Antonio Bianco

TSH doesn't do anything. None of the symptoms of hypothyroidism can be attributed to changes in TSH. It has to work through the thyroid gland.

Antonio Bianco

Most T3 in the brain does not come from the blood. It comes from being produced locally through the type 2 deiodinases.

Antonio Bianco

Mortality is 2.5 greater in the patients taking levothyroxine with hypothyroidism.

Antonio Bianco

All symptoms of hypothyroidism are not pathognomonic, meaning they're not specific for hypothyroidism. They can be caused by anything, by other diseases, by comorbidities.

Antonio Bianco

After 50 years of age, your TSH will increase by one point. Your upper limit of normal will increase by one point every 10 years.

Antonio Bianco
8 days
T4 half-life Circulating inactive pro-hormone
12 hours
T3 half-life Circulating active hormone
80%
D2 contribution to T3 production Of T3 made outside the thyroid gland
20%
D1 contribution to T3 production Of T3 made outside the thyroid gland
1,000-fold more
D2 affinity for T4 vs. D1 D2 is a more efficient enzyme
10-fold
T3 increase in brown fat upon cold exposure Observed in rat studies within a few hours, not systemic
4% to 5%
Prevalence of hypothyroidism in adults Approximately 20 million patients in the U.S.
30-40%
Remission rate for Graves' disease with medical treatment After 1-3 years of antithyroid drugs
Higher than 10
TSH level for typical hypothyroidism diagnosis With reduced free T4, for primary hypothyroidism
1 in 2,500 to 3,000
Prevalence of congenital hypothyroidism Live births
60%
Patients with positive TPO antibodies in Hashimoto's disease The remaining 40% may have other causes or unidentified antibodies
2.5 times greater
Increased mortality in hypothyroid patients on levothyroxine Compared to healthy individuals, retrospectively observed, often due to cardiometabolic diseases
30%
Mortality reduction with combination T4/T3 therapy vs. T4 monotherapy Observed in a study of 90,000 patients, relative reduction
150 micrograms
Normal daily iodine intake for adults Recommended intake
250 micrograms
Normal daily iodine intake for pregnant women Recommended intake due to expanded pool
500 to 600 micrograms
Typical daily iodine intake in Japan Due to normal diet, associated with increased autoimmune thyroid disease
10 to 1
Female to male incidence ratio of hypothyroidism Women are significantly more affected
Plus/minus 5%
Levothyroxine potency requirement FDA requirement for potency over the shelf life of the medicine