#375 - Ketogenic diet, ketosis & hyperbaric oxygen: metabolic therapies for weight loss, cognition, Alzheimer's & more | Dom D'Agostino, Ph.D.

Dec 8, 2025 Episode Page ↗
Overview

Dom D'Agostino, a neuroscientist and professor, discusses metabolic therapies including ketogenic diets, exogenous ketones, and hyperbaric oxygen. He defines nutritional versus supplemental ketosis, outlines practical ways to achieve it, and explores its therapeutic potential for conditions like cancer, neurodegenerative diseases, and psychiatric disorders.

At a Glance
21 Insights
2h 8m Duration
15 Topics
9 Concepts

Deep Dive Analysis

Dom D'Agostino's Scientific Journey into Ketosis

Navy's Interest in Oxygen Toxicity and Rebreather Diving

Defining Nutritional Ketosis and Its Historical Use

Dom's Personal Ketogenic Diet Experience and Lessons Learned

Common Mistakes and Guidance for Ketogenic Diets

Metabolic Implications and Efficacy of the Carnivore Diet

Evolution of Exogenous Ketones: Butanediol and Esters

Ketone Salts vs. Esters: Differences and Applications

Understanding D- and L-Beta-Hydroxybutyrate Enantiomers

NAD's Role in Ketosis and Challenges with Supplementation

Emerging Applications of Ketogenic Diets for Psychiatric Disorders

Cognitive and Performance Benefits of Ketone Supplementation

Ketogenic Metabolic Therapy Framework for Cancer (Glioblastoma)

Ketogenic Therapies for Alzheimer's Disease and Dementia

Hyperbaric Oxygen Therapy for Brain Injuries and Cognitive Function

Central Nervous System Oxygen Toxicity

This condition manifests as seizures when breathing high concentrations of oxygen at depth, primarily due to excessive glutamate release, activation of AMPA and NMDA receptors, inhibition of glutamic acid decarboxylase (GAD), and a burst of reactive oxygen species in the brain.

Nutritional Ketosis

A physiological state achieved through fasting or a carbohydrate-restricted, high-fat diet, characterized by blood beta-hydroxybutyrate levels above 0.5 millimoles per liter. In this state, the body primarily uses ketone bodies for energy, especially for the brain.

Pleiotropic Effects

A term used to describe the multiple beneficial biochemical, physiological, and neuropharmacological mechanisms that are activated by ketogenic diets. These diverse effects contribute to the diet's therapeutic efficacy across various conditions.

Ketone Esters

Ketone bodies (like beta-hydroxybutyrate or acetoacetate) that are covalently bound to an alcohol, such as 1,3-butanediol or glycerol. When consumed, they are designed to rapidly and significantly elevate blood ketone levels, but can sometimes cause an insulin response or metabolic stress.

Ketone Salts

Ketone bodies (specifically beta-hydroxybutyrate) that are ionically bound to mineral cations like sodium, potassium, calcium, or magnesium. They elevate blood ketones more slowly and to a lesser extent than esters, without causing an insulin spike, and also provide essential electrolytes.

Racemic Ketones (DL-BHB)

A mixture containing both D-beta-hydroxybutyrate (the naturally occurring, rapidly metabolized form for energy) and L-beta-hydroxybutyrate (the mirror image, which is metabolized slower and appears to have important signaling effects, such as inhibiting neuroinflammation).

Glucose Ketone Index (GKI)

A biomarker used to assess the depth of ketosis and metabolic health, calculated as the ratio of blood glucose (in millimoles per liter) to blood ketones (in millimoles per liter). Lower GKI values indicate a deeper state of ketosis.

Reductive Stress

A condition that can occur when D-beta-hydroxybutyrate levels rapidly spike, leading to an increase in NADH relative to NAD. This can potentially deplete NAD and cause metabolic issues, which is why a balanced redox state (e.g., with acetoacetate) is important.

Glucose Hypometabolism

A hallmark characteristic of Alzheimer's disease where the brain's capacity to utilize glucose for energy declines over time. Importantly, the brain's ability to use ketones as an alternative fuel source remains preserved in these conditions.

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Why is the Navy interested in oxygen toxicity?

Navy SEALs use closed-circuit rebreathers for stealth, breathing 100% oxygen, which at depths like 100 feet of seawater, can cause central nervous system oxygen toxicity, manifesting as seizures. Research aims to understand and prevent these incidents.

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How is nutritional ketosis defined and achieved?

Nutritional ketosis is a physiological state where blood beta-hydroxybutyrate levels are above 0.5 millimoles per liter. It's achieved by fasting or eating a carbohydrate-restricted, high-fat diet that suppresses insulin and mobilizes fatty acids for ketone production in the liver.

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How effective is a ketogenic diet for drug-resistant epilepsy?

In pediatric epilepsy, about two-thirds of patients who have failed drug therapy respond therapeutically to a ketogenic diet, with about one-third achieving complete seizure control and some even experiencing a cure. In adults, the response rate is closer to 30-40%.

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What are common mistakes people make when starting a ketogenic diet for weight loss?

Common mistakes include not tracking total calories and macronutrients, underestimating fat intake, and not monitoring blood ketone levels to correlate diet with physiological state. It's also important to get blood work done to track lipid and insulin levels.

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Can a carnivore diet induce ketosis and what are its potential benefits?

Yes, a carnivore diet, especially one focused on fatty cuts of meat and restricted calories, can induce ketosis. It may be therapeutically beneficial for individuals with autoimmune disorders due to its extreme elimination diet nature, providing all necessary micronutrients if eating nose-to-tail.

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What are the key differences between ketone esters and ketone salts?

Ketone esters are covalently bound and lead to rapid, high ketone elevation, potentially causing an insulin response and metabolic stress. Ketone salts are ionically bound to minerals, providing a slower, lower ketone rise without an insulin spike, and also supply electrolytes, making them generally safer and more practical for most people.

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Why is L-beta-hydroxybutyrate (L-BHB) important in racemic ketone supplements?

While D-BHB is rapidly metabolized for energy, L-BHB is metabolized slower and appears to have important signaling effects, such as inhibiting neuroinflammation and contributing to epigenetic changes. This prolongs the beneficial effects of ketones and makes racemic mixtures advantageous for sustained ketone elevation.

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Are higher ketone levels always better for health or performance?

No, excessively high ketone levels (above 2-3 millimolar from supplements) can lead to 'energy toxicity,' causing counter-regulatory insulin release, increased kidney burden, and blood acidification, which can be problematic and even dangerous in animal models.

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What is the role of a ketogenic diet in treating glioblastoma?

For glioblastoma, a ketogenic diet is proposed as an adjuvant therapy, aiming to achieve and maintain a Glucose Ketone Index (GKI) of 1-4. This creates an energy crisis in cancer cells when combined with drugs that aggressively target glucose and glutamine metabolism, potentially extending survival and improving quality of life.

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How might ketogenic therapies help with Alzheimer's disease?

Ketogenic therapies address glucose hypometabolism, a hallmark of Alzheimer's, by providing an alternative fuel (ketones) to the brain. They also suppress systemic and neuroinflammation, improve glucose metabolism, and increase overall brain energy metabolism, especially for patients with significant glucose hypometabolism.

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What is the recommended hyperbaric oxygen therapy protocol for acute concussion?

For acute concussion within 48-72 hours, a recommended protocol is 2 atmospheres of oxygen for 60-90 minutes, 3-5 days a week, for a total of 40 sessions. This is a significant time commitment but may offer potential benefits.

1. Prioritize Protein Intake

Ensure adequate protein intake on a ketogenic diet, especially if active or aiming for muscle gain, potentially doubling the recommended daily allowance. This prevents muscle loss and supports higher protein turnover.

2. Track Diet & Biomarkers

Track total calorie intake, macronutrient ratios, and blood ketone levels (or urine/breath) to correlate diet with physiological state. Additionally, regularly monitor lipids (triglycerides, HDL, ApoB, LDL), hemoglobin A1C, and insulin levels to assess metabolic health and manage potential adverse effects.

3. Mitigate Keto Flu with Electrolytes

Use ketone electrolytes (e.g., KetoStart) when initiating a ketogenic diet to replenish sodium and other electrolytes. This helps mitigate ‘keto flu’ symptoms, brain fog, and plasma volume contraction caused by the diet’s natriuretic and diuretic effects.

4. Gradual Keto Diet Transition

Transition to a ketogenic diet gradually over four to six weeks rather than abruptly. This approach improves adherence and can lead to more favorable health responses, potentially avoiding negative blood work or electrolyte issues.

5. High-Fiber Carb Choices

When consuming carbohydrates on a ketogenic diet, prioritize high-fiber sources like berries, broccoli, asparagus, and dark chocolate. Fiber helps prevent glycemic spikes and supports ketosis.

6. Situational Fasting Strategy

Employ fasting situationally for specific benefits rather than as a daily default. It can be particularly effective for managing inflammation events (e.g., shingles, GI issues), enhancing cognitive sharpness during demanding work, or adapting to travel.

7. Consider Carnivore for Autoimmune

For individuals with autoimmune disorders, consider a carnivore diet as an ultimate elimination diet. If following, ensure nose-to-tail eating to cover micronutrient needs (e.g., Vitamin C from liver) and consider magnesium supplementation.

8. Target GKI for Cancer Therapy

For metabolic therapy in cancer (e.g., glioblastoma), aim to achieve and maintain a Glucose Ketone Index (GKI) of 1 to 4. This GKI range sets the stage for other modalities to work by creating an energy crisis in cancer cells.

9. Ketone Salts for Supplementation

Prefer ketone salts (e.g., KetoStart by Audacious Nutrition) for exogenous ketone supplementation over ketone esters or 1,3-butanediol. Ketone salts do not cause an insulin spike, are generally safer as natural buffers, and provide sustained ketone delivery, especially when combined with MCT.

10. Optimal Exogenous Ketone Levels

When using exogenous ketones, aim to elevate blood beta-hydroxybutyrate levels to a ‘sweet spot’ of one to two millimolar. This range provides a significant increase in brain energy without risking energy toxicity or triggering counter-regulatory insulin responses.

11. Cognitive Enhancement Stack

For cognitive enhancement and sustained focus, consider a combination of alpha-GPC, MCT, caffeine, and theanine (e.g., in a product like Ketobrain). This stack provides energy, focus, and a calming effect for demanding cognitive tasks.

12. HBOT for Acute Concussion

For acute concussion, especially within the first 48-72 hours, consider hyperbaric oxygen therapy (HBOT) at 2 atmospheres for 60-90 minutes, 3-5 days a week, for up to 40 sessions. A milder protocol (1.3 atmospheres, 3 times/week for 2 weeks) can be considered for mild concussions.

13. Consult Dietitian for Clinical Keto

If using a ketogenic diet to manage a clinical condition, work with a knowledgeable registered dietitian. This ensures proper guidance and management of the therapeutic diet.

14. Reintroduce Carbs Slowly

If reintroducing carbohydrates after a period of low-carb eating, titrate them back in slowly, starting with fibrous vegetables. This helps avoid negative counter-regulatory effects, GI distress, and mood swings.

15. Avoid High-Dose 1,3-Butanediol

Avoid consuming large, chronic doses of 1,3-butanediol, especially if elderly or with compromised liver function. Large doses can cause liver enzyme elevation, aldehyde generation, and a narcotic (intoxicating) effect.

16. Assess Glucose Hypometabolism for AD

If considering ketogenic therapy for Alzheimer’s disease, prioritize patients who present with remarkable glucose hypometabolism in the brain. This patient selection criterion may lead to more favorable responses.

17. Comprehensive AD Metabolic Therapy

Approach Alzheimer’s disease management with a comprehensive metabolic therapy strategy that targets multiple contributing factors. This may involve a ketogenic diet or formula combined with various cofactors.

18. Use Ketones for Cognitive Deficit

Utilize ketones and other cognitive enhancers primarily in contexts of cognitive deficit or environmental stress. They tend to be most effective when addressing an existing deficit or stress rather than for already optimal function.

19. Consider Creatine Supplementation

Consider creatine supplementation, as it is used by Dom for its potential benefits on metabolic rate and performance.

20. Seek Stabilized NAD Forms

If considering NAD supplementation, seek stabilized forms that can effectively reach target tissues like muscle and brain. This may help overcome delivery problems and achieve desired benefits for performance or brain health.

21. Peter Attia Membership

Consider subscribing to Peter Attia’s premium membership for exclusive content and to deepen your knowledge of longevity science.

The ketogenic diet is indeed a magical diet in the way that it remarkably changes our physiology. And there's no other diet that exists that can, for example, manage drug-resistant seizures.

Dom D'Agostino

The salt-sensitive hypertension that you get is pretty much associated with sodium chloride.

Dom D'Agostino

Higher ketones are not advantageous and I think potentially very problematic.

Dom D'Agostino

If you elevate beta-hydroxybutyrate in the blood one millimolar, that represents a 10% increase of available energy to the brain.

Dom D'Agostino

Your genes are not your destiny.

Peter Attia

As we age, our capacity to use glucose decreases over time, but that does not happen with ketones. Our brain's ability to use ketones over time is preserved.

Dom D'Agostino

Ketogenic Diet for Weight Loss

Dom D'Agostino
  1. Track total calories and macronutrients using a reliable app (e.g., Carbon app).
  2. Aim for a high protein intake, typically 20-30% of total calories, or potentially 1 gram per pound of body weight for active individuals.
  3. Consume moderate fat to meet caloric needs after protein and carbohydrate targets.
  4. Prioritize high-fiber carbohydrates (e.g., leafy vegetables, broccoli, berries, dark chocolate) to allow for 50-100 grams of total carbs daily if one-third are fiber.
  5. Monitor blood ketone levels (e.g., with a Keto Mojo device) to correlate dietary intake with physiological ketosis.
  6. Undergo regular blood work to track key biomarkers such as triglycerides, HDL, ApoB, LDL, hemoglobin A1C, and insulin levels.
  7. Consider a gradual transition to the ketogenic diet over 4-6 weeks to improve adherence and overall health responses, especially to manage electrolyte balance.

Acute Concussion Hyperbaric Oxygen Therapy

Dom D'Agostino
  1. Initiate treatment as early as possible, ideally within the first 48 to 72 hours post-concussion.
  2. Administer 100% oxygen at a pressure of 2 atmospheres.
  3. Each session should last between 60 to 90 minutes.
  4. Perform sessions 3 to 5 days per week.
  5. Complete a minimum of 40 dives or sessions over the treatment period.
Above 0.5 millimoles per liter
Blood beta-hydroxybutyrate level for clinical ketosis Defines the physiological state of ketosis
20-30%
Protein percentage in modified ketogenic diets Compared to historical 8-12% and RDA of 0.8g/kg
50-100 grams per day
Carbohydrate intake for a high-fiber ketogenic diet If one-third of carbohydrates are fiber
Upwards of 220 grams per day
Protein intake for an active individual (220 lbs) Upper end for very active individuals to maintain muscle
4-6 hours
Duration of elevated ketones from one packet of Keto Start (racemic BHB salt) Observed in a person at rest, measured by continuous ketone monitor (only D-BHB)
10%
Increase in available brain energy for every 1 millimolar increase in blood beta-hydroxybutyrate Based on calculations from AV difference
1 to 4
Optimal Glucose Ketone Index (GKI) for cancer therapy Target for metabolic therapy, especially for glioblastoma
14
Number of FDA-approved applications for hyperbaric oxygen therapy Excluding off-label uses like TBI or concussion
2 atmospheres of oxygen
Pressure for hyperbaric oxygen therapy for concussion/brain injury Standard protocol for acute concussion
60-90 minutes
Session duration for hyperbaric oxygen therapy for concussion/brain injury Per session
3-5 days a week
Frequency of hyperbaric oxygen therapy for concussion/brain injury For acute concussion protocol
40 sessions
Total sessions for hyperbaric oxygen therapy for concussion/brain injury Minimum recommended for potential benefit
1.3 atmospheres
Maximum pressure achievable in soft hyperbaric chambers Compared to hard chambers that reach 2+ atmospheres
$50,000 to hundreds of thousands of dollars
Estimated cost of Alzheimer's antibody therapy Excluding potential side effects and accessibility issues
25%
Percentage of population heterozygous for APOE4 Increases risk for Alzheimer's disease